The Port and the Rosedale Industrial Park was constructed with all-weather concrete paved roads throughout its boundaries. The two roads connect to Mississippi Highways 1 and 8. Highway 1 runs parallel to the Mississippi River north and south and Highway 8 runs east to the Alabama state line. Highway 1 connects to the Helena, Arkansas-Mississippi River Bridge 50 miles to the north, and to the Greenville, Mississippi Highway 82, 40 miles to the south.

The new I-69 Intercontinental Highway System connecting Port Huron, Michigan with Laredo, Texas will traverse through Bolivar County and the Great River Bridge located 15 miles south of the Port will be built as the I-69 Mississippi River bridge crossing.

Two major interstate highway systems, I-55 and I-20, are within easy driving distance for long-haul truck shipments.

Major trucking lines, as well as a number of local privately owned trucking lines, are available for movement of cargo to and from the Port of Rosedale.

Railways
In 1981, the Port Commission purchased a branch short-line rail operation from the Illinois Central Railroad. Since that time, the Port Commission has operated the rail line on an "as needed" basis for rail customers. The rail line interchanges with the Columbus and Greenville Railroad outside of Greenville, Mississippi. In late 2001, the railroad was embargoed while the Port Commissioners worked on a major grant for total renovation.

Waterways
The Port is located at mile 585 A.H.P. on the lower Mississippi River. The 3.3-mile slack-water channel is maintained by the Corps of Engineers to a minimum of 9 feet draft and provides year-round service. Approximated five-day river transport from the Port of New Orleans allows cost-efficient transport of raw materials and products for export/import and for shipments to domestic markets.

The Public Terminal is operated by the Rosedale-Bolivar County Port Commission and offers services through its general cargo dock equipped with a 150-ton crawler crane; a dry-bulk loading system (truck to barge) for movement of all types of grain and other dry-bulk free-flowing commodities; and a dry-bulk unloading system (barge to truck) for movement of aggregates, fertilizers and other dry-bulk materials.

Some of the more common cargoes handled at the port docks are: